Fig. 3

ELISA analysis of serum antibody reactivity against cardiac troponin I (cTnI) in control or cTnI injected mice, measured at 3 and 8- weeks post-treatment. Absorbance values at 450 nm reflect anti-cTnI antibody levels. Comparisons between control and cTnI-treated groups at both 3 weeks (panel A) and 8 weeks (panel B) indicate increased antibody reactivity in cTnI-treated mice, with statistical significance at 8 weeks. Bars show mean ± SD, with individual data points displayed