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Table 1 Primers used in the quantitative real time-PCR

From: Effects of a novel peptide Ac-SDKP in radiation-induced coronary endothelial damage and resting myocardial blood flow

Gene

Forward Primer 5′to 3′

Reverse Primer 5′to 3′

Length mRNA (bp)

Claudin-1

GGCCAGGCTCTCTTTACTGG

AGAGGTTGTTTTCCGGGGAC

93

Claudin-3

AAGGTGTACGACTCGCTGCT

AGTCCCGGATAATGGTGTTG

247

Claudin-5

GGGCGTCCAGAGTTCAGTTT

TAACTTGCCTCGGAGTCTGC

213

JAM-2

GGCGACTTTAAAGACCGTGC

AGTTGCGGGCTTCACAGTAA

412

Occludin

GGATTGAGCCCGAGTGGAAA

GAGGTAGCACCACGTTGGAA

400

ZO-1

GGGGCCTACACTGATCAAGA

GGTCTCTGCTGGCTTGTTTC

370

  1. JAM-2 junctional adhesion molecule 2, ZO-1 zonula occludens